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mouse anti vgat  (Synaptic Systems)


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    Synaptic Systems mouse anti vgat
    Mouse Anti Vgat, supplied by Synaptic Systems, used in various techniques. Bioz Stars score: 96/100, based on 198 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+anti-vgat/131+011/pmc13400376-24-0-5
    Average 96 stars, based on 198 article reviews
    mouse anti vgat - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Immunohistochemistry:

    Article Title: Aberrant generation of dentate gyrus granule cells is associated with epileptic susceptibility in p53 conditional knockout mice
    Article Snippet: We used the following primary antibodies: Rabbit anti-Foxp2 (Abcam ab16046, 1:500); Rabbit anti-Cux1 (Proteintech 11,733-1-AP, 1:250); Rat anti-Ctip2 (AbCam ab18465, 1:250); Rabbit anti-Calbindin D-28 K (Swant CB38, 1:2000); Rabbit anti-Sox2 (Millipore ab5603, 1:200); Mouse anti-Ki-67 (BD Pharmingen 556,003, 1:50); Goat anti-Prox1 (R&D AF2727, 1:100); Guinea pig anti-Vglut1 (Millipore AB5905, 1:1000); Mouse anti-PSD95 (Thermofisher MA1-045, 1:250); Mouse anti-Vgat (Synaptic Systems 131,011, 1:1,000); Rabbit anti-GABAAα2 (Synaptic Systems 224,103, 1:500).

    Article Title: Nonapoptotic caspase-3 guides C1q-dependent synaptic phagocytosis by microglia
    Article Snippet: The following primary antibodies were used: rabbit anti-Iba1 (1:500; Wako, #019-19741), rat anti-CD68 (1:500; Bio-Rad, #MCA1957GA), guinea pig anti-VGLUT1 (1:1000; Synaptic Systems, #135 304), mouse anti-VGLUT1 (1:1000; Synaptic Systems, #135 311), guinea pig anti-VGAT (1:1000; Synaptic Systems, #131 004), mouse anti-VGAT (1:1000; Synaptic Systems, #131 011), rabbit anti-Homer1 (1:500; Synaptic Systems, #160 002), guinea pig anti-Homer1 (1:500; Synaptic Systems, #160 004), rabbit anti-Gephyrin (1:500; Synaptic Systems, #147 008), mouse anti-Reelin (1:500; MBL, #D223-3), mouse anti-GAD67 (1:500; EMD Millipore, #MAB5406B), rabbit anti-c-Fos (1:5000; Synaptic Systems, #226 008), chicken anti-GFP (1:1000; Abcam, #ab13970), rabbit anti-cleaved caspase-3 (1:400; Cell Signaling Technology, #9661), rabbit anti-cleaved caspase-7 (1:1000; Cell Signaling Technology, #8438), rabbit anti-cleaved caspase-9 (1:50; Cell Signaling Technology, #9509), rabbit anti-C1q (1:100; Abcam, #ab182451), rabbit anti-C1qA (1:200; Abcam, #ab155052), rabbit anti-human C3d (1:100; Agilent, #A0063), rat anti-mouse CD11b (1:500; Bio-Rad, #MCA711G), mouse anti-MAP2 (1:1000; EMD Millipore, #MAB378), mouse anti-βIII tubulin (1:200; Abcam, #ab78078), rabbit anti-DsRed (1:1000; Clontech, #632496), mouse anti-RFP (1:1000; MBL, #M155-3), rabbit anti-GFAP (1:1000; Sigma Aldrich, #G9269) and rabbit anti-LAMP1 (1:500; Abcam, #24170).

    Article Title: The dorsal column nuclei scale mechanical sensitivity in naive and neuropathic pain states.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit anti-Iba1 (1:1000, IHC) Abcam Ab178846; RRID:AB_2636859 Chicken anti-GFAP (1:1000, IHC) MyBioSource MBS555403; RRID:AB_3675842 Chicken anti-GFP (1:1000, IHC) Aves GFP 1020; RRID:AB_2307313 Rabbit anti-GFP (1:1000, IHC) Invitrogen A11122; RRID:AB_221569 Mouse anti-NeuN (1:2000, IHC) Millipore MAB377; RRID:AB_2298772 Rabbit anti-Neuropeptide Y (1:1000, IHC) Peninsula Laboratories T-4070.0050; RRID:AB_518504 Rabbit anti-dsRed (1:2000, IHC) Takara 632496; RRID:AB_10013483 Rabbit anti-cfos (1:1000, IHC) Synaptic Systems 226003; RRID:AB_2231974 Goat anti-CTb (1:5000, IHC) List Biological Labs 703; RRID:AB_10013220 Mouse anti-VGAT (1:2000, IHC) Synaptic Systems 131011; RRID:AB_887872 Guinea pig anti-VGLUT1 (1:2000, IHC) Millipore AB5905; RRID:AB_2301751 Rat anti-substance P (1:1000, IHC) Bio-Rad 8450-0505; RRID:AB_2200292 Bacterial and viral strains pAAV-hSyn-DIO-EGFP (AAV1) Addgene 50457-AAV1 pAAV1-hSyn-DIO-hM4D(Gi)-mCherry (AAV1) Addgene 44362-AAV1 pENN.AAV.hSyn.Cre.WPRE.hGH (AAVrg) Addgene 105553-AAVrg AAV phSyn1(S)-FLEX-tdTomato-T2A-SypEGFPWPRE (AAV1) Addgene 51509-AAV1 pENN.AAV.CB7.CI.eGFP.WPRE.rBG (AAV9) Addgene 105542-AAV9 pENN.AAV.CB7.CI.mCherry.WPRE.RBG (AAV9) Addgene 105544-AAV9 pAAV-hSyn-EGFP (AAV1) Addgene 50465-AAV1 Chemicals, peptides, and recombinant proteins Paclitaxel Sigma PHR1803 Strychnine hydrochloride Sigma S8753 Bicuculline methochloride Alomone B-137 CNQX disodium salt Alomone C-141 Tetrodotoxin (with citrate) Cayman Chemical 18660-81-6 4-Aminopyridine Cayman Chemical 504-24-5 Experimental Models: Organisms/Strains Mouse: AdvillinCre Jackson Laboratories Jax#032536 Mouse: AdvillinFlpO Zimmerman et al.146 Mouse: Cdx2Cre Jackson Laboratories Jax#009350 Mouse: Emx1IRES-Cre Jackson Laboratories Jax#005628 Mouse: Gad2Cherry Jackson Laboratories Jax#023140 (Continued on next page) Cell Reports 44, 115556, April 22, 2025 21

    Article Title: The transcription regulator Lmo3 is required for the development of medial ganglionic eminence derived neurons in the external globus pallidus.
    Article Snippet: The external globus pallidus (GPe) is an essential component of the basal ganglia, a group of subcortical nuclei that are involved in control of action.. Changes in the firing of GPe neurons are associated with both passive and active body movements.. Aberrant activity of GPe neurons has been linked to motor symptoms of a variety of movement disorders, such as Parkinson’s Disease, Huntington’s disease and dystonia.

    Article Title: STON2 variations are involved in synaptic dysfunction and schizophrenia-like behaviors by regulating Syt1 trafficking.
    Article Snippet: The following antibodies were used for Western blotting and immunostaining: rabbit anti-Ston2 (Sigma), rabbit anti–a-tubulin (Abcam), rabbit anti-GFP (Invitrogen), mouse anti-synaptotagmin 1 (Synaptic Systems, Cat. No. 105011), rabbit anti-SV2A (Synaptic Systems), rabbit anti-EPS15 (Sigma), mouse anti-synaptophysin (Synaptic Systems), rabbit anti-HA (Cell Signaling Technology), mouse anti-c-Myc (Santa Cruz), rabbit anti-clathrin HC (Cell Signaling Technology), anti-EPS15 (Sigma), rabbit anti-vGlut1 (Synaptic systems), mouse anti-vGat (Synaptic Systems), rabbit anti-p-AP2M1 (Cell Signaling Technology), rabbit anti-Na+-K+ ATPase (Cell Signaling Technology), anti-phosphoserine (Millipore), and mouse anti-Flag (Sigma).

    Article Title: Nausea-induced suppression of feeding is mediated by central amygdala Dlk1-expressing neurons.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Chicken polyclonal anti-beta Galactosidase (anti-LacZ) Abcam Cat#ab9361; RRID: AB_307210 Chicken polyclonal anti-GFP Thermofischer scientific Cat#A10262; RRID: AB_2534023 Rabbit anti-cFos Cell Signaling Technology Cat#2250; RRID: AB_2247211 Goat polyclonal anti-CGRP Abcam Ca# ab36001; RRID: AB_725807 Rabbit anti-calretinin (Calb2) swant Ca#CR7697; RRID: AB_2619710 Mouse anti-VGAT Synaptic Systems Ca#131011; RRID: AB_887869 Alexa Fluor 488 Donkey Anti-Rabbit Jackson ImmunoResearch Cat#711-545-152; RRID: AB_2313584 Alexa Fluor 488 Donkey Anti-Goat Jackson ImmunoResearch Cat#705-545-003; RRID: AB_2340428 Alexa Fluor 488 Donkey Anti-Chicken Jackson ImmunoResearch Cat#703-545-155; RRID: AB_2340375 Cy3 Donkey Anti-Goat Jackson ImmunoResearch Cat#705-165-147; RRID: AB_2307351 Cy3 Donkey Anti-Rabbit Jackson ImmunoResearch Cat#711-165-152; RRID: AB_2307443 Cy3 Donkey Anti-Chicken Jackson ImmunoResearch Cat#703-165-155; RRID: AB_2340363 Alexa Fluor 647 Donkey Anti-Chicken Jackson ImmunoResearch Cat#703-605-155; RRID: AB_2340379 Alexa Fluor 647 Donkey Anti-Goat Jackson ImmunoResearch Cat#705-605-147; RRID: AB_2340437 Alexa Fluor 647 Donkey Anti-Rabbit Jackson ImmunoResearch Cat#711-605-152; RRID: AB_2492288 Bacterial and virus strains AAV9-pAAV-hSyn-DIO-hM3D(Gq)-mCherry Krashes et al.51 Addgene 44361-AAV9 AAV9-pAAV-hSyn-DIO-hM4D(Gi)-mCherry Krashes et al.51 Addgene 44362-AAV9 AAV2-pAAV-hSyn-DIO-mCherry Bryan Roth Addgene 50459-AAV2 AAV5-Ef1a-DIO-ChR2-EYFP Mattis et al.52 Addgene 35509-AAV5 AAV5-Ef1a-DIO-EGFP Karl Deisseroth Addgene 27056-AAV5 AAV5-EF1a-DIO-eArch3.0-EYFP Karl Deisseroth UNC vector core AAV5-EF1a-fDIO-hChR2(H134R)-EYFP-WPRE Karl Deisseroth UNC vector core AAV-synP-DIO-STPEPB Ian Wickersham UNC vector core EnvA G-deleted rabies Karl-Klaus Conzelmann Gene Center Munich, LMU Chemicals, peptides, and recombinant proteins Triton X-100 CarlRoth Cat#3051 NaCl 0.9% Braun Cat#21222452 Clozapine-n-oxide (CNO) Sigma-Aldrich Cat#C0832-5MG Isoflurane Cp-pharma AP/DRUGS/220/96 Rimadyl (carprofen) Zoetis Cat#1939541 (Continued on next page) Cell Reports 43, 113990, April 23, 2024 17

    Article Title: Altered firing output of VIP interneurons and early dysfunctions in CA1 hippocampal circuits in the 3xTg mouse model of Alzheimer’s disease
    Article Snippet: The following primary antibodies were used in this study: goat anti-CR (1:1000; Santa Cruz, #sc-11644), chicken anti-GFP (1:1000; Aves, #GFP-1020), mouse anti-β-amyloid, 1-16 (6E10) (1:3000; Covance, #SIG-39320), anti-human PHF-tau (1:1000; Thermo Scientific, #MN1020), mouse anti-VGAT (1:500; Synaptic Systems, #131011).

    Article Title: SynCAM1 deficiency in the hippocampal parvalbumin interneurons contributes to sevoflurane-induced cognitive impairment in neonatal rats.
    Article Snippet: After washing with PBS, the sections were blocked with PBS containing 5% normal goat serum and 0.3% Triton X- 100 for 1 h at room temperature and then incubated overnight at 4°C with primary antibodies: chicken antisynCAM1 (1:1000, CM004- 3, MBL), rabbit anti- PV (1:400, ab181086, Abcam), mouse anti- PV (1:400, MAB1572, Sigma), mouse anti- GAD67 (1:400, ab26116, Abcam), guinea pig anti- Bassoon (1:500, 141004, Synaptic Systems), rabbit anti- Homer1 (1:500, 160003, Synaptic Systems) or mouse anti- vGat (1:400, 131011, Synaptic Systems), rabbit anti- SST (1:500, GTX133119, GeneTex), and rabbit anti- VIP (1:500, 63269, Cell signaling).

    Bioprocessing:

    Article Title: Aberrant generation of dentate gyrus granule cells is associated with epileptic susceptibility in p53 conditional knockout mice
    Article Snippet: We used the following primary antibodies: Rabbit anti-Foxp2 (Abcam ab16046, 1:500); Rabbit anti-Cux1 (Proteintech 11,733-1-AP, 1:250); Rat anti-Ctip2 (AbCam ab18465, 1:250); Rabbit anti-Calbindin D-28 K (Swant CB38, 1:2000); Rabbit anti-Sox2 (Millipore ab5603, 1:200); Mouse anti-Ki-67 (BD Pharmingen 556,003, 1:50); Goat anti-Prox1 (R&D AF2727, 1:100); Guinea pig anti-Vglut1 (Millipore AB5905, 1:1000); Mouse anti-PSD95 (Thermofisher MA1-045, 1:250); Mouse anti-Vgat (Synaptic Systems 131,011, 1:1,000); Rabbit anti-GABAAα2 (Synaptic Systems 224,103, 1:500).

    Article Title: Nonapoptotic caspase-3 guides C1q-dependent synaptic phagocytosis by microglia
    Article Snippet: The following primary antibodies were used: rabbit anti-Iba1 (1:500; Wako, #019-19741), rat anti-CD68 (1:500; Bio-Rad, #MCA1957GA), guinea pig anti-VGLUT1 (1:1000; Synaptic Systems, #135 304), mouse anti-VGLUT1 (1:1000; Synaptic Systems, #135 311), guinea pig anti-VGAT (1:1000; Synaptic Systems, #131 004), mouse anti-VGAT (1:1000; Synaptic Systems, #131 011), rabbit anti-Homer1 (1:500; Synaptic Systems, #160 002), guinea pig anti-Homer1 (1:500; Synaptic Systems, #160 004), rabbit anti-Gephyrin (1:500; Synaptic Systems, #147 008), mouse anti-Reelin (1:500; MBL, #D223-3), mouse anti-GAD67 (1:500; EMD Millipore, #MAB5406B), rabbit anti-c-Fos (1:5000; Synaptic Systems, #226 008), chicken anti-GFP (1:1000; Abcam, #ab13970), rabbit anti-cleaved caspase-3 (1:400; Cell Signaling Technology, #9661), rabbit anti-cleaved caspase-7 (1:1000; Cell Signaling Technology, #8438), rabbit anti-cleaved caspase-9 (1:50; Cell Signaling Technology, #9509), rabbit anti-C1q (1:100; Abcam, #ab182451), rabbit anti-C1qA (1:200; Abcam, #ab155052), rabbit anti-human C3d (1:100; Agilent, #A0063), rat anti-mouse CD11b (1:500; Bio-Rad, #MCA711G), mouse anti-MAP2 (1:1000; EMD Millipore, #MAB378), mouse anti-βIII tubulin (1:200; Abcam, #ab78078), rabbit anti-DsRed (1:1000; Clontech, #632496), mouse anti-RFP (1:1000; MBL, #M155-3), rabbit anti-GFAP (1:1000; Sigma Aldrich, #G9269) and rabbit anti-LAMP1 (1:500; Abcam, #24170).

    Article Title: The dorsal column nuclei scale mechanical sensitivity in naive and neuropathic pain states.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit anti-Iba1 (1:1000, IHC) Abcam Ab178846; RRID:AB_2636859 Chicken anti-GFAP (1:1000, IHC) MyBioSource MBS555403; RRID:AB_3675842 Chicken anti-GFP (1:1000, IHC) Aves GFP 1020; RRID:AB_2307313 Rabbit anti-GFP (1:1000, IHC) Invitrogen A11122; RRID:AB_221569 Mouse anti-NeuN (1:2000, IHC) Millipore MAB377; RRID:AB_2298772 Rabbit anti-Neuropeptide Y (1:1000, IHC) Peninsula Laboratories T-4070.0050; RRID:AB_518504 Rabbit anti-dsRed (1:2000, IHC) Takara 632496; RRID:AB_10013483 Rabbit anti-cfos (1:1000, IHC) Synaptic Systems 226003; RRID:AB_2231974 Goat anti-CTb (1:5000, IHC) List Biological Labs 703; RRID:AB_10013220 Mouse anti-VGAT (1:2000, IHC) Synaptic Systems 131011; RRID:AB_887872 Guinea pig anti-VGLUT1 (1:2000, IHC) Millipore AB5905; RRID:AB_2301751 Rat anti-substance P (1:1000, IHC) Bio-Rad 8450-0505; RRID:AB_2200292 Bacterial and viral strains pAAV-hSyn-DIO-EGFP (AAV1) Addgene 50457-AAV1 pAAV1-hSyn-DIO-hM4D(Gi)-mCherry (AAV1) Addgene 44362-AAV1 pENN.AAV.hSyn.Cre.WPRE.hGH (AAVrg) Addgene 105553-AAVrg AAV phSyn1(S)-FLEX-tdTomato-T2A-SypEGFPWPRE (AAV1) Addgene 51509-AAV1 pENN.AAV.CB7.CI.eGFP.WPRE.rBG (AAV9) Addgene 105542-AAV9 pENN.AAV.CB7.CI.mCherry.WPRE.RBG (AAV9) Addgene 105544-AAV9 pAAV-hSyn-EGFP (AAV1) Addgene 50465-AAV1 Chemicals, peptides, and recombinant proteins Paclitaxel Sigma PHR1803 Strychnine hydrochloride Sigma S8753 Bicuculline methochloride Alomone B-137 CNQX disodium salt Alomone C-141 Tetrodotoxin (with citrate) Cayman Chemical 18660-81-6 4-Aminopyridine Cayman Chemical 504-24-5 Experimental Models: Organisms/Strains Mouse: AdvillinCre Jackson Laboratories Jax#032536 Mouse: AdvillinFlpO Zimmerman et al.146 Mouse: Cdx2Cre Jackson Laboratories Jax#009350 Mouse: Emx1IRES-Cre Jackson Laboratories Jax#005628 Mouse: Gad2Cherry Jackson Laboratories Jax#023140 (Continued on next page) Cell Reports 44, 115556, April 22, 2025 21

    Article Title: The transcription regulator Lmo3 is required for the development of medial ganglionic eminence derived neurons in the external globus pallidus.
    Article Snippet: The external globus pallidus (GPe) is an essential component of the basal ganglia, a group of subcortical nuclei that are involved in control of action.. Changes in the firing of GPe neurons are associated with both passive and active body movements.. Aberrant activity of GPe neurons has been linked to motor symptoms of a variety of movement disorders, such as Parkinson’s Disease, Huntington’s disease and dystonia.

    Article Title: STON2 variations are involved in synaptic dysfunction and schizophrenia-like behaviors by regulating Syt1 trafficking.
    Article Snippet: The following antibodies were used for Western blotting and immunostaining: rabbit anti-Ston2 (Sigma), rabbit anti–a-tubulin (Abcam), rabbit anti-GFP (Invitrogen), mouse anti-synaptotagmin 1 (Synaptic Systems, Cat. No. 105011), rabbit anti-SV2A (Synaptic Systems), rabbit anti-EPS15 (Sigma), mouse anti-synaptophysin (Synaptic Systems), rabbit anti-HA (Cell Signaling Technology), mouse anti-c-Myc (Santa Cruz), rabbit anti-clathrin HC (Cell Signaling Technology), anti-EPS15 (Sigma), rabbit anti-vGlut1 (Synaptic systems), mouse anti-vGat (Synaptic Systems), rabbit anti-p-AP2M1 (Cell Signaling Technology), rabbit anti-Na+-K+ ATPase (Cell Signaling Technology), anti-phosphoserine (Millipore), and mouse anti-Flag (Sigma).

    Article Title: Nausea-induced suppression of feeding is mediated by central amygdala Dlk1-expressing neurons.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Chicken polyclonal anti-beta Galactosidase (anti-LacZ) Abcam Cat#ab9361; RRID: AB_307210 Chicken polyclonal anti-GFP Thermofischer scientific Cat#A10262; RRID: AB_2534023 Rabbit anti-cFos Cell Signaling Technology Cat#2250; RRID: AB_2247211 Goat polyclonal anti-CGRP Abcam Ca# ab36001; RRID: AB_725807 Rabbit anti-calretinin (Calb2) swant Ca#CR7697; RRID: AB_2619710 Mouse anti-VGAT Synaptic Systems Ca#131011; RRID: AB_887869 Alexa Fluor 488 Donkey Anti-Rabbit Jackson ImmunoResearch Cat#711-545-152; RRID: AB_2313584 Alexa Fluor 488 Donkey Anti-Goat Jackson ImmunoResearch Cat#705-545-003; RRID: AB_2340428 Alexa Fluor 488 Donkey Anti-Chicken Jackson ImmunoResearch Cat#703-545-155; RRID: AB_2340375 Cy3 Donkey Anti-Goat Jackson ImmunoResearch Cat#705-165-147; RRID: AB_2307351 Cy3 Donkey Anti-Rabbit Jackson ImmunoResearch Cat#711-165-152; RRID: AB_2307443 Cy3 Donkey Anti-Chicken Jackson ImmunoResearch Cat#703-165-155; RRID: AB_2340363 Alexa Fluor 647 Donkey Anti-Chicken Jackson ImmunoResearch Cat#703-605-155; RRID: AB_2340379 Alexa Fluor 647 Donkey Anti-Goat Jackson ImmunoResearch Cat#705-605-147; RRID: AB_2340437 Alexa Fluor 647 Donkey Anti-Rabbit Jackson ImmunoResearch Cat#711-605-152; RRID: AB_2492288 Bacterial and virus strains AAV9-pAAV-hSyn-DIO-hM3D(Gq)-mCherry Krashes et al.51 Addgene 44361-AAV9 AAV9-pAAV-hSyn-DIO-hM4D(Gi)-mCherry Krashes et al.51 Addgene 44362-AAV9 AAV2-pAAV-hSyn-DIO-mCherry Bryan Roth Addgene 50459-AAV2 AAV5-Ef1a-DIO-ChR2-EYFP Mattis et al.52 Addgene 35509-AAV5 AAV5-Ef1a-DIO-EGFP Karl Deisseroth Addgene 27056-AAV5 AAV5-EF1a-DIO-eArch3.0-EYFP Karl Deisseroth UNC vector core AAV5-EF1a-fDIO-hChR2(H134R)-EYFP-WPRE Karl Deisseroth UNC vector core AAV-synP-DIO-STPEPB Ian Wickersham UNC vector core EnvA G-deleted rabies Karl-Klaus Conzelmann Gene Center Munich, LMU Chemicals, peptides, and recombinant proteins Triton X-100 CarlRoth Cat#3051 NaCl 0.9% Braun Cat#21222452 Clozapine-n-oxide (CNO) Sigma-Aldrich Cat#C0832-5MG Isoflurane Cp-pharma AP/DRUGS/220/96 Rimadyl (carprofen) Zoetis Cat#1939541 (Continued on next page) Cell Reports 43, 113990, April 23, 2024 17

    Article Title: Altered firing output of VIP interneurons and early dysfunctions in CA1 hippocampal circuits in the 3xTg mouse model of Alzheimer’s disease
    Article Snippet: The following primary antibodies were used in this study: goat anti-CR (1:1000; Santa Cruz, #sc-11644), chicken anti-GFP (1:1000; Aves, #GFP-1020), mouse anti-β-amyloid, 1-16 (6E10) (1:3000; Covance, #SIG-39320), anti-human PHF-tau (1:1000; Thermo Scientific, #MN1020), mouse anti-VGAT (1:500; Synaptic Systems, #131011).

    Article Title: SynCAM1 deficiency in the hippocampal parvalbumin interneurons contributes to sevoflurane-induced cognitive impairment in neonatal rats.
    Article Snippet: After washing with PBS, the sections were blocked with PBS containing 5% normal goat serum and 0.3% Triton X- 100 for 1 h at room temperature and then incubated overnight at 4°C with primary antibodies: chicken antisynCAM1 (1:1000, CM004- 3, MBL), rabbit anti- PV (1:400, ab181086, Abcam), mouse anti- PV (1:400, MAB1572, Sigma), mouse anti- GAD67 (1:400, ab26116, Abcam), guinea pig anti- Bassoon (1:500, 141004, Synaptic Systems), rabbit anti- Homer1 (1:500, 160003, Synaptic Systems) or mouse anti- vGat (1:400, 131011, Synaptic Systems), rabbit anti- SST (1:500, GTX133119, GeneTex), and rabbit anti- VIP (1:500, 63269, Cell signaling).

    Recombinant:

    Article Title: Aberrant generation of dentate gyrus granule cells is associated with epileptic susceptibility in p53 conditional knockout mice
    Article Snippet: We used the following primary antibodies: Rabbit anti-Foxp2 (Abcam ab16046, 1:500); Rabbit anti-Cux1 (Proteintech 11,733-1-AP, 1:250); Rat anti-Ctip2 (AbCam ab18465, 1:250); Rabbit anti-Calbindin D-28 K (Swant CB38, 1:2000); Rabbit anti-Sox2 (Millipore ab5603, 1:200); Mouse anti-Ki-67 (BD Pharmingen 556,003, 1:50); Goat anti-Prox1 (R&D AF2727, 1:100); Guinea pig anti-Vglut1 (Millipore AB5905, 1:1000); Mouse anti-PSD95 (Thermofisher MA1-045, 1:250); Mouse anti-Vgat (Synaptic Systems 131,011, 1:1,000); Rabbit anti-GABAAα2 (Synaptic Systems 224,103, 1:500).

    Article Title: Nonapoptotic caspase-3 guides C1q-dependent synaptic phagocytosis by microglia
    Article Snippet: The following primary antibodies were used: rabbit anti-Iba1 (1:500; Wako, #019-19741), rat anti-CD68 (1:500; Bio-Rad, #MCA1957GA), guinea pig anti-VGLUT1 (1:1000; Synaptic Systems, #135 304), mouse anti-VGLUT1 (1:1000; Synaptic Systems, #135 311), guinea pig anti-VGAT (1:1000; Synaptic Systems, #131 004), mouse anti-VGAT (1:1000; Synaptic Systems, #131 011), rabbit anti-Homer1 (1:500; Synaptic Systems, #160 002), guinea pig anti-Homer1 (1:500; Synaptic Systems, #160 004), rabbit anti-Gephyrin (1:500; Synaptic Systems, #147 008), mouse anti-Reelin (1:500; MBL, #D223-3), mouse anti-GAD67 (1:500; EMD Millipore, #MAB5406B), rabbit anti-c-Fos (1:5000; Synaptic Systems, #226 008), chicken anti-GFP (1:1000; Abcam, #ab13970), rabbit anti-cleaved caspase-3 (1:400; Cell Signaling Technology, #9661), rabbit anti-cleaved caspase-7 (1:1000; Cell Signaling Technology, #8438), rabbit anti-cleaved caspase-9 (1:50; Cell Signaling Technology, #9509), rabbit anti-C1q (1:100; Abcam, #ab182451), rabbit anti-C1qA (1:200; Abcam, #ab155052), rabbit anti-human C3d (1:100; Agilent, #A0063), rat anti-mouse CD11b (1:500; Bio-Rad, #MCA711G), mouse anti-MAP2 (1:1000; EMD Millipore, #MAB378), mouse anti-βIII tubulin (1:200; Abcam, #ab78078), rabbit anti-DsRed (1:1000; Clontech, #632496), mouse anti-RFP (1:1000; MBL, #M155-3), rabbit anti-GFAP (1:1000; Sigma Aldrich, #G9269) and rabbit anti-LAMP1 (1:500; Abcam, #24170).

    Article Title: The dorsal column nuclei scale mechanical sensitivity in naive and neuropathic pain states.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit anti-Iba1 (1:1000, IHC) Abcam Ab178846; RRID:AB_2636859 Chicken anti-GFAP (1:1000, IHC) MyBioSource MBS555403; RRID:AB_3675842 Chicken anti-GFP (1:1000, IHC) Aves GFP 1020; RRID:AB_2307313 Rabbit anti-GFP (1:1000, IHC) Invitrogen A11122; RRID:AB_221569 Mouse anti-NeuN (1:2000, IHC) Millipore MAB377; RRID:AB_2298772 Rabbit anti-Neuropeptide Y (1:1000, IHC) Peninsula Laboratories T-4070.0050; RRID:AB_518504 Rabbit anti-dsRed (1:2000, IHC) Takara 632496; RRID:AB_10013483 Rabbit anti-cfos (1:1000, IHC) Synaptic Systems 226003; RRID:AB_2231974 Goat anti-CTb (1:5000, IHC) List Biological Labs 703; RRID:AB_10013220 Mouse anti-VGAT (1:2000, IHC) Synaptic Systems 131011; RRID:AB_887872 Guinea pig anti-VGLUT1 (1:2000, IHC) Millipore AB5905; RRID:AB_2301751 Rat anti-substance P (1:1000, IHC) Bio-Rad 8450-0505; RRID:AB_2200292 Bacterial and viral strains pAAV-hSyn-DIO-EGFP (AAV1) Addgene 50457-AAV1 pAAV1-hSyn-DIO-hM4D(Gi)-mCherry (AAV1) Addgene 44362-AAV1 pENN.AAV.hSyn.Cre.WPRE.hGH (AAVrg) Addgene 105553-AAVrg AAV phSyn1(S)-FLEX-tdTomato-T2A-SypEGFPWPRE (AAV1) Addgene 51509-AAV1 pENN.AAV.CB7.CI.eGFP.WPRE.rBG (AAV9) Addgene 105542-AAV9 pENN.AAV.CB7.CI.mCherry.WPRE.RBG (AAV9) Addgene 105544-AAV9 pAAV-hSyn-EGFP (AAV1) Addgene 50465-AAV1 Chemicals, peptides, and recombinant proteins Paclitaxel Sigma PHR1803 Strychnine hydrochloride Sigma S8753 Bicuculline methochloride Alomone B-137 CNQX disodium salt Alomone C-141 Tetrodotoxin (with citrate) Cayman Chemical 18660-81-6 4-Aminopyridine Cayman Chemical 504-24-5 Experimental Models: Organisms/Strains Mouse: AdvillinCre Jackson Laboratories Jax#032536 Mouse: AdvillinFlpO Zimmerman et al.146 Mouse: Cdx2Cre Jackson Laboratories Jax#009350 Mouse: Emx1IRES-Cre Jackson Laboratories Jax#005628 Mouse: Gad2Cherry Jackson Laboratories Jax#023140 (Continued on next page) Cell Reports 44, 115556, April 22, 2025 21

    Article Title: The transcription regulator Lmo3 is required for the development of medial ganglionic eminence derived neurons in the external globus pallidus.
    Article Snippet: The external globus pallidus (GPe) is an essential component of the basal ganglia, a group of subcortical nuclei that are involved in control of action.. Changes in the firing of GPe neurons are associated with both passive and active body movements.. Aberrant activity of GPe neurons has been linked to motor symptoms of a variety of movement disorders, such as Parkinson’s Disease, Huntington’s disease and dystonia.

    Article Title: STON2 variations are involved in synaptic dysfunction and schizophrenia-like behaviors by regulating Syt1 trafficking.
    Article Snippet: The following antibodies were used for Western blotting and immunostaining: rabbit anti-Ston2 (Sigma), rabbit anti–a-tubulin (Abcam), rabbit anti-GFP (Invitrogen), mouse anti-synaptotagmin 1 (Synaptic Systems, Cat. No. 105011), rabbit anti-SV2A (Synaptic Systems), rabbit anti-EPS15 (Sigma), mouse anti-synaptophysin (Synaptic Systems), rabbit anti-HA (Cell Signaling Technology), mouse anti-c-Myc (Santa Cruz), rabbit anti-clathrin HC (Cell Signaling Technology), anti-EPS15 (Sigma), rabbit anti-vGlut1 (Synaptic systems), mouse anti-vGat (Synaptic Systems), rabbit anti-p-AP2M1 (Cell Signaling Technology), rabbit anti-Na+-K+ ATPase (Cell Signaling Technology), anti-phosphoserine (Millipore), and mouse anti-Flag (Sigma).

    Article Title: Nausea-induced suppression of feeding is mediated by central amygdala Dlk1-expressing neurons.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Chicken polyclonal anti-beta Galactosidase (anti-LacZ) Abcam Cat#ab9361; RRID: AB_307210 Chicken polyclonal anti-GFP Thermofischer scientific Cat#A10262; RRID: AB_2534023 Rabbit anti-cFos Cell Signaling Technology Cat#2250; RRID: AB_2247211 Goat polyclonal anti-CGRP Abcam Ca# ab36001; RRID: AB_725807 Rabbit anti-calretinin (Calb2) swant Ca#CR7697; RRID: AB_2619710 Mouse anti-VGAT Synaptic Systems Ca#131011; RRID: AB_887869 Alexa Fluor 488 Donkey Anti-Rabbit Jackson ImmunoResearch Cat#711-545-152; RRID: AB_2313584 Alexa Fluor 488 Donkey Anti-Goat Jackson ImmunoResearch Cat#705-545-003; RRID: AB_2340428 Alexa Fluor 488 Donkey Anti-Chicken Jackson ImmunoResearch Cat#703-545-155; RRID: AB_2340375 Cy3 Donkey Anti-Goat Jackson ImmunoResearch Cat#705-165-147; RRID: AB_2307351 Cy3 Donkey Anti-Rabbit Jackson ImmunoResearch Cat#711-165-152; RRID: AB_2307443 Cy3 Donkey Anti-Chicken Jackson ImmunoResearch Cat#703-165-155; RRID: AB_2340363 Alexa Fluor 647 Donkey Anti-Chicken Jackson ImmunoResearch Cat#703-605-155; RRID: AB_2340379 Alexa Fluor 647 Donkey Anti-Goat Jackson ImmunoResearch Cat#705-605-147; RRID: AB_2340437 Alexa Fluor 647 Donkey Anti-Rabbit Jackson ImmunoResearch Cat#711-605-152; RRID: AB_2492288 Bacterial and virus strains AAV9-pAAV-hSyn-DIO-hM3D(Gq)-mCherry Krashes et al.51 Addgene 44361-AAV9 AAV9-pAAV-hSyn-DIO-hM4D(Gi)-mCherry Krashes et al.51 Addgene 44362-AAV9 AAV2-pAAV-hSyn-DIO-mCherry Bryan Roth Addgene 50459-AAV2 AAV5-Ef1a-DIO-ChR2-EYFP Mattis et al.52 Addgene 35509-AAV5 AAV5-Ef1a-DIO-EGFP Karl Deisseroth Addgene 27056-AAV5 AAV5-EF1a-DIO-eArch3.0-EYFP Karl Deisseroth UNC vector core AAV5-EF1a-fDIO-hChR2(H134R)-EYFP-WPRE Karl Deisseroth UNC vector core AAV-synP-DIO-STPEPB Ian Wickersham UNC vector core EnvA G-deleted rabies Karl-Klaus Conzelmann Gene Center Munich, LMU Chemicals, peptides, and recombinant proteins Triton X-100 CarlRoth Cat#3051 NaCl 0.9% Braun Cat#21222452 Clozapine-n-oxide (CNO) Sigma-Aldrich Cat#C0832-5MG Isoflurane Cp-pharma AP/DRUGS/220/96 Rimadyl (carprofen) Zoetis Cat#1939541 (Continued on next page) Cell Reports 43, 113990, April 23, 2024 17

    Article Title: Altered firing output of VIP interneurons and early dysfunctions in CA1 hippocampal circuits in the 3xTg mouse model of Alzheimer’s disease
    Article Snippet: The following primary antibodies were used in this study: goat anti-CR (1:1000; Santa Cruz, #sc-11644), chicken anti-GFP (1:1000; Aves, #GFP-1020), mouse anti-β-amyloid, 1-16 (6E10) (1:3000; Covance, #SIG-39320), anti-human PHF-tau (1:1000; Thermo Scientific, #MN1020), mouse anti-VGAT (1:500; Synaptic Systems, #131011).

    Article Title: SynCAM1 deficiency in the hippocampal parvalbumin interneurons contributes to sevoflurane-induced cognitive impairment in neonatal rats.
    Article Snippet: After washing with PBS, the sections were blocked with PBS containing 5% normal goat serum and 0.3% Triton X- 100 for 1 h at room temperature and then incubated overnight at 4°C with primary antibodies: chicken antisynCAM1 (1:1000, CM004- 3, MBL), rabbit anti- PV (1:400, ab181086, Abcam), mouse anti- PV (1:400, MAB1572, Sigma), mouse anti- GAD67 (1:400, ab26116, Abcam), guinea pig anti- Bassoon (1:500, 141004, Synaptic Systems), rabbit anti- Homer1 (1:500, 160003, Synaptic Systems) or mouse anti- vGat (1:400, 131011, Synaptic Systems), rabbit anti- SST (1:500, GTX133119, GeneTex), and rabbit anti- VIP (1:500, 63269, Cell signaling).

    Virus:

    Article Title: Aberrant generation of dentate gyrus granule cells is associated with epileptic susceptibility in p53 conditional knockout mice
    Article Snippet: We used the following primary antibodies: Rabbit anti-Foxp2 (Abcam ab16046, 1:500); Rabbit anti-Cux1 (Proteintech 11,733-1-AP, 1:250); Rat anti-Ctip2 (AbCam ab18465, 1:250); Rabbit anti-Calbindin D-28 K (Swant CB38, 1:2000); Rabbit anti-Sox2 (Millipore ab5603, 1:200); Mouse anti-Ki-67 (BD Pharmingen 556,003, 1:50); Goat anti-Prox1 (R&D AF2727, 1:100); Guinea pig anti-Vglut1 (Millipore AB5905, 1:1000); Mouse anti-PSD95 (Thermofisher MA1-045, 1:250); Mouse anti-Vgat (Synaptic Systems 131,011, 1:1,000); Rabbit anti-GABAAα2 (Synaptic Systems 224,103, 1:500).

    Article Title: Nonapoptotic caspase-3 guides C1q-dependent synaptic phagocytosis by microglia
    Article Snippet: The following primary antibodies were used: rabbit anti-Iba1 (1:500; Wako, #019-19741), rat anti-CD68 (1:500; Bio-Rad, #MCA1957GA), guinea pig anti-VGLUT1 (1:1000; Synaptic Systems, #135 304), mouse anti-VGLUT1 (1:1000; Synaptic Systems, #135 311), guinea pig anti-VGAT (1:1000; Synaptic Systems, #131 004), mouse anti-VGAT (1:1000; Synaptic Systems, #131 011), rabbit anti-Homer1 (1:500; Synaptic Systems, #160 002), guinea pig anti-Homer1 (1:500; Synaptic Systems, #160 004), rabbit anti-Gephyrin (1:500; Synaptic Systems, #147 008), mouse anti-Reelin (1:500; MBL, #D223-3), mouse anti-GAD67 (1:500; EMD Millipore, #MAB5406B), rabbit anti-c-Fos (1:5000; Synaptic Systems, #226 008), chicken anti-GFP (1:1000; Abcam, #ab13970), rabbit anti-cleaved caspase-3 (1:400; Cell Signaling Technology, #9661), rabbit anti-cleaved caspase-7 (1:1000; Cell Signaling Technology, #8438), rabbit anti-cleaved caspase-9 (1:50; Cell Signaling Technology, #9509), rabbit anti-C1q (1:100; Abcam, #ab182451), rabbit anti-C1qA (1:200; Abcam, #ab155052), rabbit anti-human C3d (1:100; Agilent, #A0063), rat anti-mouse CD11b (1:500; Bio-Rad, #MCA711G), mouse anti-MAP2 (1:1000; EMD Millipore, #MAB378), mouse anti-βIII tubulin (1:200; Abcam, #ab78078), rabbit anti-DsRed (1:1000; Clontech, #632496), mouse anti-RFP (1:1000; MBL, #M155-3), rabbit anti-GFAP (1:1000; Sigma Aldrich, #G9269) and rabbit anti-LAMP1 (1:500; Abcam, #24170).

    Article Title: The dorsal column nuclei scale mechanical sensitivity in naive and neuropathic pain states.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit anti-Iba1 (1:1000, IHC) Abcam Ab178846; RRID:AB_2636859 Chicken anti-GFAP (1:1000, IHC) MyBioSource MBS555403; RRID:AB_3675842 Chicken anti-GFP (1:1000, IHC) Aves GFP 1020; RRID:AB_2307313 Rabbit anti-GFP (1:1000, IHC) Invitrogen A11122; RRID:AB_221569 Mouse anti-NeuN (1:2000, IHC) Millipore MAB377; RRID:AB_2298772 Rabbit anti-Neuropeptide Y (1:1000, IHC) Peninsula Laboratories T-4070.0050; RRID:AB_518504 Rabbit anti-dsRed (1:2000, IHC) Takara 632496; RRID:AB_10013483 Rabbit anti-cfos (1:1000, IHC) Synaptic Systems 226003; RRID:AB_2231974 Goat anti-CTb (1:5000, IHC) List Biological Labs 703; RRID:AB_10013220 Mouse anti-VGAT (1:2000, IHC) Synaptic Systems 131011; RRID:AB_887872 Guinea pig anti-VGLUT1 (1:2000, IHC) Millipore AB5905; RRID:AB_2301751 Rat anti-substance P (1:1000, IHC) Bio-Rad 8450-0505; RRID:AB_2200292 Bacterial and viral strains pAAV-hSyn-DIO-EGFP (AAV1) Addgene 50457-AAV1 pAAV1-hSyn-DIO-hM4D(Gi)-mCherry (AAV1) Addgene 44362-AAV1 pENN.AAV.hSyn.Cre.WPRE.hGH (AAVrg) Addgene 105553-AAVrg AAV phSyn1(S)-FLEX-tdTomato-T2A-SypEGFPWPRE (AAV1) Addgene 51509-AAV1 pENN.AAV.CB7.CI.eGFP.WPRE.rBG (AAV9) Addgene 105542-AAV9 pENN.AAV.CB7.CI.mCherry.WPRE.RBG (AAV9) Addgene 105544-AAV9 pAAV-hSyn-EGFP (AAV1) Addgene 50465-AAV1 Chemicals, peptides, and recombinant proteins Paclitaxel Sigma PHR1803 Strychnine hydrochloride Sigma S8753 Bicuculline methochloride Alomone B-137 CNQX disodium salt Alomone C-141 Tetrodotoxin (with citrate) Cayman Chemical 18660-81-6 4-Aminopyridine Cayman Chemical 504-24-5 Experimental Models: Organisms/Strains Mouse: AdvillinCre Jackson Laboratories Jax#032536 Mouse: AdvillinFlpO Zimmerman et al.146 Mouse: Cdx2Cre Jackson Laboratories Jax#009350 Mouse: Emx1IRES-Cre Jackson Laboratories Jax#005628 Mouse: Gad2Cherry Jackson Laboratories Jax#023140 (Continued on next page) Cell Reports 44, 115556, April 22, 2025 21

    Article Title: The transcription regulator Lmo3 is required for the development of medial ganglionic eminence derived neurons in the external globus pallidus.
    Article Snippet: The external globus pallidus (GPe) is an essential component of the basal ganglia, a group of subcortical nuclei that are involved in control of action.. Changes in the firing of GPe neurons are associated with both passive and active body movements.. Aberrant activity of GPe neurons has been linked to motor symptoms of a variety of movement disorders, such as Parkinson’s Disease, Huntington’s disease and dystonia.

    Article Title: STON2 variations are involved in synaptic dysfunction and schizophrenia-like behaviors by regulating Syt1 trafficking.
    Article Snippet: The following antibodies were used for Western blotting and immunostaining: rabbit anti-Ston2 (Sigma), rabbit anti–a-tubulin (Abcam), rabbit anti-GFP (Invitrogen), mouse anti-synaptotagmin 1 (Synaptic Systems, Cat. No. 105011), rabbit anti-SV2A (Synaptic Systems), rabbit anti-EPS15 (Sigma), mouse anti-synaptophysin (Synaptic Systems), rabbit anti-HA (Cell Signaling Technology), mouse anti-c-Myc (Santa Cruz), rabbit anti-clathrin HC (Cell Signaling Technology), anti-EPS15 (Sigma), rabbit anti-vGlut1 (Synaptic systems), mouse anti-vGat (Synaptic Systems), rabbit anti-p-AP2M1 (Cell Signaling Technology), rabbit anti-Na+-K+ ATPase (Cell Signaling Technology), anti-phosphoserine (Millipore), and mouse anti-Flag (Sigma).

    Article Title: Nausea-induced suppression of feeding is mediated by central amygdala Dlk1-expressing neurons.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Chicken polyclonal anti-beta Galactosidase (anti-LacZ) Abcam Cat#ab9361; RRID: AB_307210 Chicken polyclonal anti-GFP Thermofischer scientific Cat#A10262; RRID: AB_2534023 Rabbit anti-cFos Cell Signaling Technology Cat#2250; RRID: AB_2247211 Goat polyclonal anti-CGRP Abcam Ca# ab36001; RRID: AB_725807 Rabbit anti-calretinin (Calb2) swant Ca#CR7697; RRID: AB_2619710 Mouse anti-VGAT Synaptic Systems Ca#131011; RRID: AB_887869 Alexa Fluor 488 Donkey Anti-Rabbit Jackson ImmunoResearch Cat#711-545-152; RRID: AB_2313584 Alexa Fluor 488 Donkey Anti-Goat Jackson ImmunoResearch Cat#705-545-003; RRID: AB_2340428 Alexa Fluor 488 Donkey Anti-Chicken Jackson ImmunoResearch Cat#703-545-155; RRID: AB_2340375 Cy3 Donkey Anti-Goat Jackson ImmunoResearch Cat#705-165-147; RRID: AB_2307351 Cy3 Donkey Anti-Rabbit Jackson ImmunoResearch Cat#711-165-152; RRID: AB_2307443 Cy3 Donkey Anti-Chicken Jackson ImmunoResearch Cat#703-165-155; RRID: AB_2340363 Alexa Fluor 647 Donkey Anti-Chicken Jackson ImmunoResearch Cat#703-605-155; RRID: AB_2340379 Alexa Fluor 647 Donkey Anti-Goat Jackson ImmunoResearch Cat#705-605-147; RRID: AB_2340437 Alexa Fluor 647 Donkey Anti-Rabbit Jackson ImmunoResearch Cat#711-605-152; RRID: AB_2492288 Bacterial and virus strains AAV9-pAAV-hSyn-DIO-hM3D(Gq)-mCherry Krashes et al.51 Addgene 44361-AAV9 AAV9-pAAV-hSyn-DIO-hM4D(Gi)-mCherry Krashes et al.51 Addgene 44362-AAV9 AAV2-pAAV-hSyn-DIO-mCherry Bryan Roth Addgene 50459-AAV2 AAV5-Ef1a-DIO-ChR2-EYFP Mattis et al.52 Addgene 35509-AAV5 AAV5-Ef1a-DIO-EGFP Karl Deisseroth Addgene 27056-AAV5 AAV5-EF1a-DIO-eArch3.0-EYFP Karl Deisseroth UNC vector core AAV5-EF1a-fDIO-hChR2(H134R)-EYFP-WPRE Karl Deisseroth UNC vector core AAV-synP-DIO-STPEPB Ian Wickersham UNC vector core EnvA G-deleted rabies Karl-Klaus Conzelmann Gene Center Munich, LMU Chemicals, peptides, and recombinant proteins Triton X-100 CarlRoth Cat#3051 NaCl 0.9% Braun Cat#21222452 Clozapine-n-oxide (CNO) Sigma-Aldrich Cat#C0832-5MG Isoflurane Cp-pharma AP/DRUGS/220/96 Rimadyl (carprofen) Zoetis Cat#1939541 (Continued on next page) Cell Reports 43, 113990, April 23, 2024 17

    Article Title: Altered firing output of VIP interneurons and early dysfunctions in CA1 hippocampal circuits in the 3xTg mouse model of Alzheimer’s disease
    Article Snippet: The following primary antibodies were used in this study: goat anti-CR (1:1000; Santa Cruz, #sc-11644), chicken anti-GFP (1:1000; Aves, #GFP-1020), mouse anti-β-amyloid, 1-16 (6E10) (1:3000; Covance, #SIG-39320), anti-human PHF-tau (1:1000; Thermo Scientific, #MN1020), mouse anti-VGAT (1:500; Synaptic Systems, #131011).

    Article Title: SynCAM1 deficiency in the hippocampal parvalbumin interneurons contributes to sevoflurane-induced cognitive impairment in neonatal rats.
    Article Snippet: After washing with PBS, the sections were blocked with PBS containing 5% normal goat serum and 0.3% Triton X- 100 for 1 h at room temperature and then incubated overnight at 4°C with primary antibodies: chicken antisynCAM1 (1:1000, CM004- 3, MBL), rabbit anti- PV (1:400, ab181086, Abcam), mouse anti- PV (1:400, MAB1572, Sigma), mouse anti- GAD67 (1:400, ab26116, Abcam), guinea pig anti- Bassoon (1:500, 141004, Synaptic Systems), rabbit anti- Homer1 (1:500, 160003, Synaptic Systems) or mouse anti- vGat (1:400, 131011, Synaptic Systems), rabbit anti- SST (1:500, GTX133119, GeneTex), and rabbit anti- VIP (1:500, 63269, Cell signaling).

    Plasmid Preparation:

    Article Title: Aberrant generation of dentate gyrus granule cells is associated with epileptic susceptibility in p53 conditional knockout mice
    Article Snippet: We used the following primary antibodies: Rabbit anti-Foxp2 (Abcam ab16046, 1:500); Rabbit anti-Cux1 (Proteintech 11,733-1-AP, 1:250); Rat anti-Ctip2 (AbCam ab18465, 1:250); Rabbit anti-Calbindin D-28 K (Swant CB38, 1:2000); Rabbit anti-Sox2 (Millipore ab5603, 1:200); Mouse anti-Ki-67 (BD Pharmingen 556,003, 1:50); Goat anti-Prox1 (R&D AF2727, 1:100); Guinea pig anti-Vglut1 (Millipore AB5905, 1:1000); Mouse anti-PSD95 (Thermofisher MA1-045, 1:250); Mouse anti-Vgat (Synaptic Systems 131,011, 1:1,000); Rabbit anti-GABAAα2 (Synaptic Systems 224,103, 1:500).

    Article Title: Nonapoptotic caspase-3 guides C1q-dependent synaptic phagocytosis by microglia
    Article Snippet: The following primary antibodies were used: rabbit anti-Iba1 (1:500; Wako, #019-19741), rat anti-CD68 (1:500; Bio-Rad, #MCA1957GA), guinea pig anti-VGLUT1 (1:1000; Synaptic Systems, #135 304), mouse anti-VGLUT1 (1:1000; Synaptic Systems, #135 311), guinea pig anti-VGAT (1:1000; Synaptic Systems, #131 004), mouse anti-VGAT (1:1000; Synaptic Systems, #131 011), rabbit anti-Homer1 (1:500; Synaptic Systems, #160 002), guinea pig anti-Homer1 (1:500; Synaptic Systems, #160 004), rabbit anti-Gephyrin (1:500; Synaptic Systems, #147 008), mouse anti-Reelin (1:500; MBL, #D223-3), mouse anti-GAD67 (1:500; EMD Millipore, #MAB5406B), rabbit anti-c-Fos (1:5000; Synaptic Systems, #226 008), chicken anti-GFP (1:1000; Abcam, #ab13970), rabbit anti-cleaved caspase-3 (1:400; Cell Signaling Technology, #9661), rabbit anti-cleaved caspase-7 (1:1000; Cell Signaling Technology, #8438), rabbit anti-cleaved caspase-9 (1:50; Cell Signaling Technology, #9509), rabbit anti-C1q (1:100; Abcam, #ab182451), rabbit anti-C1qA (1:200; Abcam, #ab155052), rabbit anti-human C3d (1:100; Agilent, #A0063), rat anti-mouse CD11b (1:500; Bio-Rad, #MCA711G), mouse anti-MAP2 (1:1000; EMD Millipore, #MAB378), mouse anti-βIII tubulin (1:200; Abcam, #ab78078), rabbit anti-DsRed (1:1000; Clontech, #632496), mouse anti-RFP (1:1000; MBL, #M155-3), rabbit anti-GFAP (1:1000; Sigma Aldrich, #G9269) and rabbit anti-LAMP1 (1:500; Abcam, #24170).

    Article Title: The dorsal column nuclei scale mechanical sensitivity in naive and neuropathic pain states.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit anti-Iba1 (1:1000, IHC) Abcam Ab178846; RRID:AB_2636859 Chicken anti-GFAP (1:1000, IHC) MyBioSource MBS555403; RRID:AB_3675842 Chicken anti-GFP (1:1000, IHC) Aves GFP 1020; RRID:AB_2307313 Rabbit anti-GFP (1:1000, IHC) Invitrogen A11122; RRID:AB_221569 Mouse anti-NeuN (1:2000, IHC) Millipore MAB377; RRID:AB_2298772 Rabbit anti-Neuropeptide Y (1:1000, IHC) Peninsula Laboratories T-4070.0050; RRID:AB_518504 Rabbit anti-dsRed (1:2000, IHC) Takara 632496; RRID:AB_10013483 Rabbit anti-cfos (1:1000, IHC) Synaptic Systems 226003; RRID:AB_2231974 Goat anti-CTb (1:5000, IHC) List Biological Labs 703; RRID:AB_10013220 Mouse anti-VGAT (1:2000, IHC) Synaptic Systems 131011; RRID:AB_887872 Guinea pig anti-VGLUT1 (1:2000, IHC) Millipore AB5905; RRID:AB_2301751 Rat anti-substance P (1:1000, IHC) Bio-Rad 8450-0505; RRID:AB_2200292 Bacterial and viral strains pAAV-hSyn-DIO-EGFP (AAV1) Addgene 50457-AAV1 pAAV1-hSyn-DIO-hM4D(Gi)-mCherry (AAV1) Addgene 44362-AAV1 pENN.AAV.hSyn.Cre.WPRE.hGH (AAVrg) Addgene 105553-AAVrg AAV phSyn1(S)-FLEX-tdTomato-T2A-SypEGFPWPRE (AAV1) Addgene 51509-AAV1 pENN.AAV.CB7.CI.eGFP.WPRE.rBG (AAV9) Addgene 105542-AAV9 pENN.AAV.CB7.CI.mCherry.WPRE.RBG (AAV9) Addgene 105544-AAV9 pAAV-hSyn-EGFP (AAV1) Addgene 50465-AAV1 Chemicals, peptides, and recombinant proteins Paclitaxel Sigma PHR1803 Strychnine hydrochloride Sigma S8753 Bicuculline methochloride Alomone B-137 CNQX disodium salt Alomone C-141 Tetrodotoxin (with citrate) Cayman Chemical 18660-81-6 4-Aminopyridine Cayman Chemical 504-24-5 Experimental Models: Organisms/Strains Mouse: AdvillinCre Jackson Laboratories Jax#032536 Mouse: AdvillinFlpO Zimmerman et al.146 Mouse: Cdx2Cre Jackson Laboratories Jax#009350 Mouse: Emx1IRES-Cre Jackson Laboratories Jax#005628 Mouse: Gad2Cherry Jackson Laboratories Jax#023140 (Continued on next page) Cell Reports 44, 115556, April 22, 2025 21

    Article Title: The transcription regulator Lmo3 is required for the development of medial ganglionic eminence derived neurons in the external globus pallidus.
    Article Snippet: The external globus pallidus (GPe) is an essential component of the basal ganglia, a group of subcortical nuclei that are involved in control of action.. Changes in the firing of GPe neurons are associated with both passive and active body movements.. Aberrant activity of GPe neurons has been linked to motor symptoms of a variety of movement disorders, such as Parkinson’s Disease, Huntington’s disease and dystonia.

    Article Title: STON2 variations are involved in synaptic dysfunction and schizophrenia-like behaviors by regulating Syt1 trafficking.
    Article Snippet: The following antibodies were used for Western blotting and immunostaining: rabbit anti-Ston2 (Sigma), rabbit anti–a-tubulin (Abcam), rabbit anti-GFP (Invitrogen), mouse anti-synaptotagmin 1 (Synaptic Systems, Cat. No. 105011), rabbit anti-SV2A (Synaptic Systems), rabbit anti-EPS15 (Sigma), mouse anti-synaptophysin (Synaptic Systems), rabbit anti-HA (Cell Signaling Technology), mouse anti-c-Myc (Santa Cruz), rabbit anti-clathrin HC (Cell Signaling Technology), anti-EPS15 (Sigma), rabbit anti-vGlut1 (Synaptic systems), mouse anti-vGat (Synaptic Systems), rabbit anti-p-AP2M1 (Cell Signaling Technology), rabbit anti-Na+-K+ ATPase (Cell Signaling Technology), anti-phosphoserine (Millipore), and mouse anti-Flag (Sigma).

    Article Title: Nausea-induced suppression of feeding is mediated by central amygdala Dlk1-expressing neurons.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Chicken polyclonal anti-beta Galactosidase (anti-LacZ) Abcam Cat#ab9361; RRID: AB_307210 Chicken polyclonal anti-GFP Thermofischer scientific Cat#A10262; RRID: AB_2534023 Rabbit anti-cFos Cell Signaling Technology Cat#2250; RRID: AB_2247211 Goat polyclonal anti-CGRP Abcam Ca# ab36001; RRID: AB_725807 Rabbit anti-calretinin (Calb2) swant Ca#CR7697; RRID: AB_2619710 Mouse anti-VGAT Synaptic Systems Ca#131011; RRID: AB_887869 Alexa Fluor 488 Donkey Anti-Rabbit Jackson ImmunoResearch Cat#711-545-152; RRID: AB_2313584 Alexa Fluor 488 Donkey Anti-Goat Jackson ImmunoResearch Cat#705-545-003; RRID: AB_2340428 Alexa Fluor 488 Donkey Anti-Chicken Jackson ImmunoResearch Cat#703-545-155; RRID: AB_2340375 Cy3 Donkey Anti-Goat Jackson ImmunoResearch Cat#705-165-147; RRID: AB_2307351 Cy3 Donkey Anti-Rabbit Jackson ImmunoResearch Cat#711-165-152; RRID: AB_2307443 Cy3 Donkey Anti-Chicken Jackson ImmunoResearch Cat#703-165-155; RRID: AB_2340363 Alexa Fluor 647 Donkey Anti-Chicken Jackson ImmunoResearch Cat#703-605-155; RRID: AB_2340379 Alexa Fluor 647 Donkey Anti-Goat Jackson ImmunoResearch Cat#705-605-147; RRID: AB_2340437 Alexa Fluor 647 Donkey Anti-Rabbit Jackson ImmunoResearch Cat#711-605-152; RRID: AB_2492288 Bacterial and virus strains AAV9-pAAV-hSyn-DIO-hM3D(Gq)-mCherry Krashes et al.51 Addgene 44361-AAV9 AAV9-pAAV-hSyn-DIO-hM4D(Gi)-mCherry Krashes et al.51 Addgene 44362-AAV9 AAV2-pAAV-hSyn-DIO-mCherry Bryan Roth Addgene 50459-AAV2 AAV5-Ef1a-DIO-ChR2-EYFP Mattis et al.52 Addgene 35509-AAV5 AAV5-Ef1a-DIO-EGFP Karl Deisseroth Addgene 27056-AAV5 AAV5-EF1a-DIO-eArch3.0-EYFP Karl Deisseroth UNC vector core AAV5-EF1a-fDIO-hChR2(H134R)-EYFP-WPRE Karl Deisseroth UNC vector core AAV-synP-DIO-STPEPB Ian Wickersham UNC vector core EnvA G-deleted rabies Karl-Klaus Conzelmann Gene Center Munich, LMU Chemicals, peptides, and recombinant proteins Triton X-100 CarlRoth Cat#3051 NaCl 0.9% Braun Cat#21222452 Clozapine-n-oxide (CNO) Sigma-Aldrich Cat#C0832-5MG Isoflurane Cp-pharma AP/DRUGS/220/96 Rimadyl (carprofen) Zoetis Cat#1939541 (Continued on next page) Cell Reports 43, 113990, April 23, 2024 17

    Article Title: Altered firing output of VIP interneurons and early dysfunctions in CA1 hippocampal circuits in the 3xTg mouse model of Alzheimer’s disease
    Article Snippet: The following primary antibodies were used in this study: goat anti-CR (1:1000; Santa Cruz, #sc-11644), chicken anti-GFP (1:1000; Aves, #GFP-1020), mouse anti-β-amyloid, 1-16 (6E10) (1:3000; Covance, #SIG-39320), anti-human PHF-tau (1:1000; Thermo Scientific, #MN1020), mouse anti-VGAT (1:500; Synaptic Systems, #131011).

    Article Title: SynCAM1 deficiency in the hippocampal parvalbumin interneurons contributes to sevoflurane-induced cognitive impairment in neonatal rats.
    Article Snippet: After washing with PBS, the sections were blocked with PBS containing 5% normal goat serum and 0.3% Triton X- 100 for 1 h at room temperature and then incubated overnight at 4°C with primary antibodies: chicken antisynCAM1 (1:1000, CM004- 3, MBL), rabbit anti- PV (1:400, ab181086, Abcam), mouse anti- PV (1:400, MAB1572, Sigma), mouse anti- GAD67 (1:400, ab26116, Abcam), guinea pig anti- Bassoon (1:500, 141004, Synaptic Systems), rabbit anti- Homer1 (1:500, 160003, Synaptic Systems) or mouse anti- vGat (1:400, 131011, Synaptic Systems), rabbit anti- SST (1:500, GTX133119, GeneTex), and rabbit anti- VIP (1:500, 63269, Cell signaling).

    Western Blot:

    Article Title: Aberrant generation of dentate gyrus granule cells is associated with epileptic susceptibility in p53 conditional knockout mice
    Article Snippet: We used the following primary antibodies: Rabbit anti-Foxp2 (Abcam ab16046, 1:500); Rabbit anti-Cux1 (Proteintech 11,733-1-AP, 1:250); Rat anti-Ctip2 (AbCam ab18465, 1:250); Rabbit anti-Calbindin D-28 K (Swant CB38, 1:2000); Rabbit anti-Sox2 (Millipore ab5603, 1:200); Mouse anti-Ki-67 (BD Pharmingen 556,003, 1:50); Goat anti-Prox1 (R&D AF2727, 1:100); Guinea pig anti-Vglut1 (Millipore AB5905, 1:1000); Mouse anti-PSD95 (Thermofisher MA1-045, 1:250); Mouse anti-Vgat (Synaptic Systems 131,011, 1:1,000); Rabbit anti-GABAAα2 (Synaptic Systems 224,103, 1:500).

    Article Title: Nonapoptotic caspase-3 guides C1q-dependent synaptic phagocytosis by microglia
    Article Snippet: The following primary antibodies were used: rabbit anti-Iba1 (1:500; Wako, #019-19741), rat anti-CD68 (1:500; Bio-Rad, #MCA1957GA), guinea pig anti-VGLUT1 (1:1000; Synaptic Systems, #135 304), mouse anti-VGLUT1 (1:1000; Synaptic Systems, #135 311), guinea pig anti-VGAT (1:1000; Synaptic Systems, #131 004), mouse anti-VGAT (1:1000; Synaptic Systems, #131 011), rabbit anti-Homer1 (1:500; Synaptic Systems, #160 002), guinea pig anti-Homer1 (1:500; Synaptic Systems, #160 004), rabbit anti-Gephyrin (1:500; Synaptic Systems, #147 008), mouse anti-Reelin (1:500; MBL, #D223-3), mouse anti-GAD67 (1:500; EMD Millipore, #MAB5406B), rabbit anti-c-Fos (1:5000; Synaptic Systems, #226 008), chicken anti-GFP (1:1000; Abcam, #ab13970), rabbit anti-cleaved caspase-3 (1:400; Cell Signaling Technology, #9661), rabbit anti-cleaved caspase-7 (1:1000; Cell Signaling Technology, #8438), rabbit anti-cleaved caspase-9 (1:50; Cell Signaling Technology, #9509), rabbit anti-C1q (1:100; Abcam, #ab182451), rabbit anti-C1qA (1:200; Abcam, #ab155052), rabbit anti-human C3d (1:100; Agilent, #A0063), rat anti-mouse CD11b (1:500; Bio-Rad, #MCA711G), mouse anti-MAP2 (1:1000; EMD Millipore, #MAB378), mouse anti-βIII tubulin (1:200; Abcam, #ab78078), rabbit anti-DsRed (1:1000; Clontech, #632496), mouse anti-RFP (1:1000; MBL, #M155-3), rabbit anti-GFAP (1:1000; Sigma Aldrich, #G9269) and rabbit anti-LAMP1 (1:500; Abcam, #24170).

    Article Title: The dorsal column nuclei scale mechanical sensitivity in naive and neuropathic pain states.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit anti-Iba1 (1:1000, IHC) Abcam Ab178846; RRID:AB_2636859 Chicken anti-GFAP (1:1000, IHC) MyBioSource MBS555403; RRID:AB_3675842 Chicken anti-GFP (1:1000, IHC) Aves GFP 1020; RRID:AB_2307313 Rabbit anti-GFP (1:1000, IHC) Invitrogen A11122; RRID:AB_221569 Mouse anti-NeuN (1:2000, IHC) Millipore MAB377; RRID:AB_2298772 Rabbit anti-Neuropeptide Y (1:1000, IHC) Peninsula Laboratories T-4070.0050; RRID:AB_518504 Rabbit anti-dsRed (1:2000, IHC) Takara 632496; RRID:AB_10013483 Rabbit anti-cfos (1:1000, IHC) Synaptic Systems 226003; RRID:AB_2231974 Goat anti-CTb (1:5000, IHC) List Biological Labs 703; RRID:AB_10013220 Mouse anti-VGAT (1:2000, IHC) Synaptic Systems 131011; RRID:AB_887872 Guinea pig anti-VGLUT1 (1:2000, IHC) Millipore AB5905; RRID:AB_2301751 Rat anti-substance P (1:1000, IHC) Bio-Rad 8450-0505; RRID:AB_2200292 Bacterial and viral strains pAAV-hSyn-DIO-EGFP (AAV1) Addgene 50457-AAV1 pAAV1-hSyn-DIO-hM4D(Gi)-mCherry (AAV1) Addgene 44362-AAV1 pENN.AAV.hSyn.Cre.WPRE.hGH (AAVrg) Addgene 105553-AAVrg AAV phSyn1(S)-FLEX-tdTomato-T2A-SypEGFPWPRE (AAV1) Addgene 51509-AAV1 pENN.AAV.CB7.CI.eGFP.WPRE.rBG (AAV9) Addgene 105542-AAV9 pENN.AAV.CB7.CI.mCherry.WPRE.RBG (AAV9) Addgene 105544-AAV9 pAAV-hSyn-EGFP (AAV1) Addgene 50465-AAV1 Chemicals, peptides, and recombinant proteins Paclitaxel Sigma PHR1803 Strychnine hydrochloride Sigma S8753 Bicuculline methochloride Alomone B-137 CNQX disodium salt Alomone C-141 Tetrodotoxin (with citrate) Cayman Chemical 18660-81-6 4-Aminopyridine Cayman Chemical 504-24-5 Experimental Models: Organisms/Strains Mouse: AdvillinCre Jackson Laboratories Jax#032536 Mouse: AdvillinFlpO Zimmerman et al.146 Mouse: Cdx2Cre Jackson Laboratories Jax#009350 Mouse: Emx1IRES-Cre Jackson Laboratories Jax#005628 Mouse: Gad2Cherry Jackson Laboratories Jax#023140 (Continued on next page) Cell Reports 44, 115556, April 22, 2025 21

    Article Title: The transcription regulator Lmo3 is required for the development of medial ganglionic eminence derived neurons in the external globus pallidus.
    Article Snippet: The external globus pallidus (GPe) is an essential component of the basal ganglia, a group of subcortical nuclei that are involved in control of action.. Changes in the firing of GPe neurons are associated with both passive and active body movements.. Aberrant activity of GPe neurons has been linked to motor symptoms of a variety of movement disorders, such as Parkinson’s Disease, Huntington’s disease and dystonia.

    Article Title: STON2 variations are involved in synaptic dysfunction and schizophrenia-like behaviors by regulating Syt1 trafficking.
    Article Snippet: The following antibodies were used for Western blotting and immunostaining: rabbit anti-Ston2 (Sigma), rabbit anti–a-tubulin (Abcam), rabbit anti-GFP (Invitrogen), mouse anti-synaptotagmin 1 (Synaptic Systems, Cat. No. 105011), rabbit anti-SV2A (Synaptic Systems), rabbit anti-EPS15 (Sigma), mouse anti-synaptophysin (Synaptic Systems), rabbit anti-HA (Cell Signaling Technology), mouse anti-c-Myc (Santa Cruz), rabbit anti-clathrin HC (Cell Signaling Technology), anti-EPS15 (Sigma), rabbit anti-vGlut1 (Synaptic systems), mouse anti-vGat (Synaptic Systems), rabbit anti-p-AP2M1 (Cell Signaling Technology), rabbit anti-Na+-K+ ATPase (Cell Signaling Technology), anti-phosphoserine (Millipore), and mouse anti-Flag (Sigma).

    Article Title: Nausea-induced suppression of feeding is mediated by central amygdala Dlk1-expressing neurons.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Chicken polyclonal anti-beta Galactosidase (anti-LacZ) Abcam Cat#ab9361; RRID: AB_307210 Chicken polyclonal anti-GFP Thermofischer scientific Cat#A10262; RRID: AB_2534023 Rabbit anti-cFos Cell Signaling Technology Cat#2250; RRID: AB_2247211 Goat polyclonal anti-CGRP Abcam Ca# ab36001; RRID: AB_725807 Rabbit anti-calretinin (Calb2) swant Ca#CR7697; RRID: AB_2619710 Mouse anti-VGAT Synaptic Systems Ca#131011; RRID: AB_887869 Alexa Fluor 488 Donkey Anti-Rabbit Jackson ImmunoResearch Cat#711-545-152; RRID: AB_2313584 Alexa Fluor 488 Donkey Anti-Goat Jackson ImmunoResearch Cat#705-545-003; RRID: AB_2340428 Alexa Fluor 488 Donkey Anti-Chicken Jackson ImmunoResearch Cat#703-545-155; RRID: AB_2340375 Cy3 Donkey Anti-Goat Jackson ImmunoResearch Cat#705-165-147; RRID: AB_2307351 Cy3 Donkey Anti-Rabbit Jackson ImmunoResearch Cat#711-165-152; RRID: AB_2307443 Cy3 Donkey Anti-Chicken Jackson ImmunoResearch Cat#703-165-155; RRID: AB_2340363 Alexa Fluor 647 Donkey Anti-Chicken Jackson ImmunoResearch Cat#703-605-155; RRID: AB_2340379 Alexa Fluor 647 Donkey Anti-Goat Jackson ImmunoResearch Cat#705-605-147; RRID: AB_2340437 Alexa Fluor 647 Donkey Anti-Rabbit Jackson ImmunoResearch Cat#711-605-152; RRID: AB_2492288 Bacterial and virus strains AAV9-pAAV-hSyn-DIO-hM3D(Gq)-mCherry Krashes et al.51 Addgene 44361-AAV9 AAV9-pAAV-hSyn-DIO-hM4D(Gi)-mCherry Krashes et al.51 Addgene 44362-AAV9 AAV2-pAAV-hSyn-DIO-mCherry Bryan Roth Addgene 50459-AAV2 AAV5-Ef1a-DIO-ChR2-EYFP Mattis et al.52 Addgene 35509-AAV5 AAV5-Ef1a-DIO-EGFP Karl Deisseroth Addgene 27056-AAV5 AAV5-EF1a-DIO-eArch3.0-EYFP Karl Deisseroth UNC vector core AAV5-EF1a-fDIO-hChR2(H134R)-EYFP-WPRE Karl Deisseroth UNC vector core AAV-synP-DIO-STPEPB Ian Wickersham UNC vector core EnvA G-deleted rabies Karl-Klaus Conzelmann Gene Center Munich, LMU Chemicals, peptides, and recombinant proteins Triton X-100 CarlRoth Cat#3051 NaCl 0.9% Braun Cat#21222452 Clozapine-n-oxide (CNO) Sigma-Aldrich Cat#C0832-5MG Isoflurane Cp-pharma AP/DRUGS/220/96 Rimadyl (carprofen) Zoetis Cat#1939541 (Continued on next page) Cell Reports 43, 113990, April 23, 2024 17

    Article Title: Altered firing output of VIP interneurons and early dysfunctions in CA1 hippocampal circuits in the 3xTg mouse model of Alzheimer’s disease
    Article Snippet: The following primary antibodies were used in this study: goat anti-CR (1:1000; Santa Cruz, #sc-11644), chicken anti-GFP (1:1000; Aves, #GFP-1020), mouse anti-β-amyloid, 1-16 (6E10) (1:3000; Covance, #SIG-39320), anti-human PHF-tau (1:1000; Thermo Scientific, #MN1020), mouse anti-VGAT (1:500; Synaptic Systems, #131011).

    Article Title: SynCAM1 deficiency in the hippocampal parvalbumin interneurons contributes to sevoflurane-induced cognitive impairment in neonatal rats.
    Article Snippet: After washing with PBS, the sections were blocked with PBS containing 5% normal goat serum and 0.3% Triton X- 100 for 1 h at room temperature and then incubated overnight at 4°C with primary antibodies: chicken antisynCAM1 (1:1000, CM004- 3, MBL), rabbit anti- PV (1:400, ab181086, Abcam), mouse anti- PV (1:400, MAB1572, Sigma), mouse anti- GAD67 (1:400, ab26116, Abcam), guinea pig anti- Bassoon (1:500, 141004, Synaptic Systems), rabbit anti- Homer1 (1:500, 160003, Synaptic Systems) or mouse anti- vGat (1:400, 131011, Synaptic Systems), rabbit anti- SST (1:500, GTX133119, GeneTex), and rabbit anti- VIP (1:500, 63269, Cell signaling).

    Immunostaining:

    Article Title: Aberrant generation of dentate gyrus granule cells is associated with epileptic susceptibility in p53 conditional knockout mice
    Article Snippet: We used the following primary antibodies: Rabbit anti-Foxp2 (Abcam ab16046, 1:500); Rabbit anti-Cux1 (Proteintech 11,733-1-AP, 1:250); Rat anti-Ctip2 (AbCam ab18465, 1:250); Rabbit anti-Calbindin D-28 K (Swant CB38, 1:2000); Rabbit anti-Sox2 (Millipore ab5603, 1:200); Mouse anti-Ki-67 (BD Pharmingen 556,003, 1:50); Goat anti-Prox1 (R&D AF2727, 1:100); Guinea pig anti-Vglut1 (Millipore AB5905, 1:1000); Mouse anti-PSD95 (Thermofisher MA1-045, 1:250); Mouse anti-Vgat (Synaptic Systems 131,011, 1:1,000); Rabbit anti-GABAAα2 (Synaptic Systems 224,103, 1:500).

    Article Title: Nonapoptotic caspase-3 guides C1q-dependent synaptic phagocytosis by microglia
    Article Snippet: The following primary antibodies were used: rabbit anti-Iba1 (1:500; Wako, #019-19741), rat anti-CD68 (1:500; Bio-Rad, #MCA1957GA), guinea pig anti-VGLUT1 (1:1000; Synaptic Systems, #135 304), mouse anti-VGLUT1 (1:1000; Synaptic Systems, #135 311), guinea pig anti-VGAT (1:1000; Synaptic Systems, #131 004), mouse anti-VGAT (1:1000; Synaptic Systems, #131 011), rabbit anti-Homer1 (1:500; Synaptic Systems, #160 002), guinea pig anti-Homer1 (1:500; Synaptic Systems, #160 004), rabbit anti-Gephyrin (1:500; Synaptic Systems, #147 008), mouse anti-Reelin (1:500; MBL, #D223-3), mouse anti-GAD67 (1:500; EMD Millipore, #MAB5406B), rabbit anti-c-Fos (1:5000; Synaptic Systems, #226 008), chicken anti-GFP (1:1000; Abcam, #ab13970), rabbit anti-cleaved caspase-3 (1:400; Cell Signaling Technology, #9661), rabbit anti-cleaved caspase-7 (1:1000; Cell Signaling Technology, #8438), rabbit anti-cleaved caspase-9 (1:50; Cell Signaling Technology, #9509), rabbit anti-C1q (1:100; Abcam, #ab182451), rabbit anti-C1qA (1:200; Abcam, #ab155052), rabbit anti-human C3d (1:100; Agilent, #A0063), rat anti-mouse CD11b (1:500; Bio-Rad, #MCA711G), mouse anti-MAP2 (1:1000; EMD Millipore, #MAB378), mouse anti-βIII tubulin (1:200; Abcam, #ab78078), rabbit anti-DsRed (1:1000; Clontech, #632496), mouse anti-RFP (1:1000; MBL, #M155-3), rabbit anti-GFAP (1:1000; Sigma Aldrich, #G9269) and rabbit anti-LAMP1 (1:500; Abcam, #24170).

    Article Title: The dorsal column nuclei scale mechanical sensitivity in naive and neuropathic pain states.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit anti-Iba1 (1:1000, IHC) Abcam Ab178846; RRID:AB_2636859 Chicken anti-GFAP (1:1000, IHC) MyBioSource MBS555403; RRID:AB_3675842 Chicken anti-GFP (1:1000, IHC) Aves GFP 1020; RRID:AB_2307313 Rabbit anti-GFP (1:1000, IHC) Invitrogen A11122; RRID:AB_221569 Mouse anti-NeuN (1:2000, IHC) Millipore MAB377; RRID:AB_2298772 Rabbit anti-Neuropeptide Y (1:1000, IHC) Peninsula Laboratories T-4070.0050; RRID:AB_518504 Rabbit anti-dsRed (1:2000, IHC) Takara 632496; RRID:AB_10013483 Rabbit anti-cfos (1:1000, IHC) Synaptic Systems 226003; RRID:AB_2231974 Goat anti-CTb (1:5000, IHC) List Biological Labs 703; RRID:AB_10013220 Mouse anti-VGAT (1:2000, IHC) Synaptic Systems 131011; RRID:AB_887872 Guinea pig anti-VGLUT1 (1:2000, IHC) Millipore AB5905; RRID:AB_2301751 Rat anti-substance P (1:1000, IHC) Bio-Rad 8450-0505; RRID:AB_2200292 Bacterial and viral strains pAAV-hSyn-DIO-EGFP (AAV1) Addgene 50457-AAV1 pAAV1-hSyn-DIO-hM4D(Gi)-mCherry (AAV1) Addgene 44362-AAV1 pENN.AAV.hSyn.Cre.WPRE.hGH (AAVrg) Addgene 105553-AAVrg AAV phSyn1(S)-FLEX-tdTomato-T2A-SypEGFPWPRE (AAV1) Addgene 51509-AAV1 pENN.AAV.CB7.CI.eGFP.WPRE.rBG (AAV9) Addgene 105542-AAV9 pENN.AAV.CB7.CI.mCherry.WPRE.RBG (AAV9) Addgene 105544-AAV9 pAAV-hSyn-EGFP (AAV1) Addgene 50465-AAV1 Chemicals, peptides, and recombinant proteins Paclitaxel Sigma PHR1803 Strychnine hydrochloride Sigma S8753 Bicuculline methochloride Alomone B-137 CNQX disodium salt Alomone C-141 Tetrodotoxin (with citrate) Cayman Chemical 18660-81-6 4-Aminopyridine Cayman Chemical 504-24-5 Experimental Models: Organisms/Strains Mouse: AdvillinCre Jackson Laboratories Jax#032536 Mouse: AdvillinFlpO Zimmerman et al.146 Mouse: Cdx2Cre Jackson Laboratories Jax#009350 Mouse: Emx1IRES-Cre Jackson Laboratories Jax#005628 Mouse: Gad2Cherry Jackson Laboratories Jax#023140 (Continued on next page) Cell Reports 44, 115556, April 22, 2025 21

    Article Title: The transcription regulator Lmo3 is required for the development of medial ganglionic eminence derived neurons in the external globus pallidus.
    Article Snippet: The external globus pallidus (GPe) is an essential component of the basal ganglia, a group of subcortical nuclei that are involved in control of action.. Changes in the firing of GPe neurons are associated with both passive and active body movements.. Aberrant activity of GPe neurons has been linked to motor symptoms of a variety of movement disorders, such as Parkinson’s Disease, Huntington’s disease and dystonia.

    Article Title: STON2 variations are involved in synaptic dysfunction and schizophrenia-like behaviors by regulating Syt1 trafficking.
    Article Snippet: The following antibodies were used for Western blotting and immunostaining: rabbit anti-Ston2 (Sigma), rabbit anti–a-tubulin (Abcam), rabbit anti-GFP (Invitrogen), mouse anti-synaptotagmin 1 (Synaptic Systems, Cat. No. 105011), rabbit anti-SV2A (Synaptic Systems), rabbit anti-EPS15 (Sigma), mouse anti-synaptophysin (Synaptic Systems), rabbit anti-HA (Cell Signaling Technology), mouse anti-c-Myc (Santa Cruz), rabbit anti-clathrin HC (Cell Signaling Technology), anti-EPS15 (Sigma), rabbit anti-vGlut1 (Synaptic systems), mouse anti-vGat (Synaptic Systems), rabbit anti-p-AP2M1 (Cell Signaling Technology), rabbit anti-Na+-K+ ATPase (Cell Signaling Technology), anti-phosphoserine (Millipore), and mouse anti-Flag (Sigma).

    Article Title: Nausea-induced suppression of feeding is mediated by central amygdala Dlk1-expressing neurons.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Chicken polyclonal anti-beta Galactosidase (anti-LacZ) Abcam Cat#ab9361; RRID: AB_307210 Chicken polyclonal anti-GFP Thermofischer scientific Cat#A10262; RRID: AB_2534023 Rabbit anti-cFos Cell Signaling Technology Cat#2250; RRID: AB_2247211 Goat polyclonal anti-CGRP Abcam Ca# ab36001; RRID: AB_725807 Rabbit anti-calretinin (Calb2) swant Ca#CR7697; RRID: AB_2619710 Mouse anti-VGAT Synaptic Systems Ca#131011; RRID: AB_887869 Alexa Fluor 488 Donkey Anti-Rabbit Jackson ImmunoResearch Cat#711-545-152; RRID: AB_2313584 Alexa Fluor 488 Donkey Anti-Goat Jackson ImmunoResearch Cat#705-545-003; RRID: AB_2340428 Alexa Fluor 488 Donkey Anti-Chicken Jackson ImmunoResearch Cat#703-545-155; RRID: AB_2340375 Cy3 Donkey Anti-Goat Jackson ImmunoResearch Cat#705-165-147; RRID: AB_2307351 Cy3 Donkey Anti-Rabbit Jackson ImmunoResearch Cat#711-165-152; RRID: AB_2307443 Cy3 Donkey Anti-Chicken Jackson ImmunoResearch Cat#703-165-155; RRID: AB_2340363 Alexa Fluor 647 Donkey Anti-Chicken Jackson ImmunoResearch Cat#703-605-155; RRID: AB_2340379 Alexa Fluor 647 Donkey Anti-Goat Jackson ImmunoResearch Cat#705-605-147; RRID: AB_2340437 Alexa Fluor 647 Donkey Anti-Rabbit Jackson ImmunoResearch Cat#711-605-152; RRID: AB_2492288 Bacterial and virus strains AAV9-pAAV-hSyn-DIO-hM3D(Gq)-mCherry Krashes et al.51 Addgene 44361-AAV9 AAV9-pAAV-hSyn-DIO-hM4D(Gi)-mCherry Krashes et al.51 Addgene 44362-AAV9 AAV2-pAAV-hSyn-DIO-mCherry Bryan Roth Addgene 50459-AAV2 AAV5-Ef1a-DIO-ChR2-EYFP Mattis et al.52 Addgene 35509-AAV5 AAV5-Ef1a-DIO-EGFP Karl Deisseroth Addgene 27056-AAV5 AAV5-EF1a-DIO-eArch3.0-EYFP Karl Deisseroth UNC vector core AAV5-EF1a-fDIO-hChR2(H134R)-EYFP-WPRE Karl Deisseroth UNC vector core AAV-synP-DIO-STPEPB Ian Wickersham UNC vector core EnvA G-deleted rabies Karl-Klaus Conzelmann Gene Center Munich, LMU Chemicals, peptides, and recombinant proteins Triton X-100 CarlRoth Cat#3051 NaCl 0.9% Braun Cat#21222452 Clozapine-n-oxide (CNO) Sigma-Aldrich Cat#C0832-5MG Isoflurane Cp-pharma AP/DRUGS/220/96 Rimadyl (carprofen) Zoetis Cat#1939541 (Continued on next page) Cell Reports 43, 113990, April 23, 2024 17

    Article Title: Altered firing output of VIP interneurons and early dysfunctions in CA1 hippocampal circuits in the 3xTg mouse model of Alzheimer’s disease
    Article Snippet: The following primary antibodies were used in this study: goat anti-CR (1:1000; Santa Cruz, #sc-11644), chicken anti-GFP (1:1000; Aves, #GFP-1020), mouse anti-β-amyloid, 1-16 (6E10) (1:3000; Covance, #SIG-39320), anti-human PHF-tau (1:1000; Thermo Scientific, #MN1020), mouse anti-VGAT (1:500; Synaptic Systems, #131011).

    Article Title: SynCAM1 deficiency in the hippocampal parvalbumin interneurons contributes to sevoflurane-induced cognitive impairment in neonatal rats.
    Article Snippet: After washing with PBS, the sections were blocked with PBS containing 5% normal goat serum and 0.3% Triton X- 100 for 1 h at room temperature and then incubated overnight at 4°C with primary antibodies: chicken antisynCAM1 (1:1000, CM004- 3, MBL), rabbit anti- PV (1:400, ab181086, Abcam), mouse anti- PV (1:400, MAB1572, Sigma), mouse anti- GAD67 (1:400, ab26116, Abcam), guinea pig anti- Bassoon (1:500, 141004, Synaptic Systems), rabbit anti- Homer1 (1:500, 160003, Synaptic Systems) or mouse anti- vGat (1:400, 131011, Synaptic Systems), rabbit anti- SST (1:500, GTX133119, GeneTex), and rabbit anti- VIP (1:500, 63269, Cell signaling).



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    mouse anti vgat primary antibody  (Synaptic Systems)
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    Immunocytochemistry of neurons cultured on a soft (0.1 kPa) and b stiff (10 kPa) substrates, showing (from top to bottom) neurofilament (NF), postsynaptic marker (NRL), vesicular glutamate transporter <t>2</t> <t>(Vglut2;</t> excitatory), vesicular GABA transporter <t>(VGAT;</t> inhibitory), and an overlay of all channels. c , d Quantification of synapse densities. Both glutamatergic and GABAergic synapse densities were lower on stiff substrates than on soft ones at DIVs 10 and 14. Boxplots showing detected synapses per mm neurofilament; p -values provided above the plots (two-tailed t-tests). Data from three independent experiments. For DIV 10, 31 fields of view (FOV) were analysed on 0.1 kPa substrates and 37 FOV on 10 kPa substrates. For DIV 14, 35 FOV were analysed on 0.1 kPa substrates and 40 FOV on 10 kPa substrates. Boxplots show the median (central line), the interquartile range (boxes), and whiskers represent 1.5 times the interquartile range. Scale bar: 5 μm.
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    Image Search Results


    Immunocytochemistry of neurons cultured on a soft (0.1 kPa) and b stiff (10 kPa) substrates, showing (from top to bottom) neurofilament (NF), postsynaptic marker (NRL), vesicular glutamate transporter 2 (Vglut2; excitatory), vesicular GABA transporter (VGAT; inhibitory), and an overlay of all channels. c , d Quantification of synapse densities. Both glutamatergic and GABAergic synapse densities were lower on stiff substrates than on soft ones at DIVs 10 and 14. Boxplots showing detected synapses per mm neurofilament; p -values provided above the plots (two-tailed t-tests). Data from three independent experiments. For DIV 10, 31 fields of view (FOV) were analysed on 0.1 kPa substrates and 37 FOV on 10 kPa substrates. For DIV 14, 35 FOV were analysed on 0.1 kPa substrates and 40 FOV on 10 kPa substrates. Boxplots show the median (central line), the interquartile range (boxes), and whiskers represent 1.5 times the interquartile range. Scale bar: 5 μm.

    Journal: Nature Communications

    Article Title: Environmental stiffness regulates neuronal maturation via Piezo1-mediated transthyretin activity

    doi: 10.1038/s41467-025-64810-3

    Figure Lengend Snippet: Immunocytochemistry of neurons cultured on a soft (0.1 kPa) and b stiff (10 kPa) substrates, showing (from top to bottom) neurofilament (NF), postsynaptic marker (NRL), vesicular glutamate transporter 2 (Vglut2; excitatory), vesicular GABA transporter (VGAT; inhibitory), and an overlay of all channels. c , d Quantification of synapse densities. Both glutamatergic and GABAergic synapse densities were lower on stiff substrates than on soft ones at DIVs 10 and 14. Boxplots showing detected synapses per mm neurofilament; p -values provided above the plots (two-tailed t-tests). Data from three independent experiments. For DIV 10, 31 fields of view (FOV) were analysed on 0.1 kPa substrates and 37 FOV on 10 kPa substrates. For DIV 14, 35 FOV were analysed on 0.1 kPa substrates and 40 FOV on 10 kPa substrates. Boxplots show the median (central line), the interquartile range (boxes), and whiskers represent 1.5 times the interquartile range. Scale bar: 5 μm.

    Article Snippet: The primary antibodies used were chicken anti-Neurofilament (abcam, ab4680, 1:1000), rabbit anti-neuroligin (Synaptic Systems #129 213, 1:1000), guinea pig Vglut2 (Synaptic Systems #135 404 1:1000), and mouse VGAT (Synaptic Systems #131 011, 1:750).

    Techniques: Immunocytochemistry, Cell Culture, Marker, Two Tailed Test